valuation of the Cytotoxic Effect of Tuibur (Tobacco Brew) in Different Cultured Cell Lines valuation of the Cytotoxic Effect of Tuibur (Tobacco Brew) in Different Cultured Cell Lines

Abstract

The consumption of tobacco and tobacco products is directly correlated to many human ailments including cancer. Tuibur.the brewed tobacco, is one of the forms of smokeless tobacco [1], which is frequently used by the inhabitants of Northeast India including Mizoram. The greater use of tobacco and its related products has led to increased frequency of cancer in this region of India. Therefore, it was decided to study the cytotoxicity of two grades of commonly used tuibur in cultured human peripheral blood lymphocytes (HPBLs), HeLa, V79 and Dalton’s lymphoma ascites (DLA) cells along with nicotine by MTT assay.Treatment of HPBLs, HeLa, V79 and DLA cells for 24 h with two grades of commercial tuibur and nicotine led to a concentration dependent rise in their cytotoxicity by MTT assay. Our results indicate that constant consumption of tuibur has a deleterious effect on cultured human peripheral blood lymphocytes, HeLa, V79 and DLA cells.