http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/793 Sat, 02 May 2026 19:52:52 GMT 2026-05-02T19:52:52Z http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/798 Title: Analysis of Chemical Constituents and Antiparasitic Activities of the Extracts of Imperata cylindrica Authors: Lalchhandama, K Abstract: With little known pharmacological potentials, Imperata cylindrica is infamous as an invasive grass. But to the Mizo people, the rhizome-root part is a convenient and potent remedy for intestinal helminthiasis. It is an acclaimed effective deworming agent against both tapeworm and roundworm infections, which makes it more interesting for the fact that antiparasitic drugs are usually active against a particular group of helminthic worms. GC-MS analysis of the chloroform extract indicated the presence of 13 volatile compounds, among which trans-13-Octadecenoic acid was by far, at relative abundance of 98.4%, the most abundant. There were 17 compounds in the petroleum ether extract with n-hexadecanoic acid at relative abundance of 99.7% is the major compound. Biologically active compounds such as 2-methoxy-4-vinylphenol, 6-methylenebicyclo [3.2.0] hept-3-en-2-one, campesterol, and stigmasterol were detected. An in vitro test showed that both the extracts were effective against the tapeworm Raillietina echinobothrida but only the chloroform at the highest concentration (20 mg/ml) was effective on the roundworm Ascaridia galli. These data imply that the plant extracts are a potential source of antiparasitic compounds. Wed, 04 Sep 2019 00:00:00 GMT http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/798 2019-09-04T00:00:00Z http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/797 Title: Anticancer and DNA-Protecting Potentials of Spilanthes acmella (Toothache Plant) Grown in Mizoram, India Authors: Lalchhandama, K Abstract: The toothache plant, Spilanthes acmella (L.) Murray, is an important culinary and medicinal plant. Here, we study its probable properties as a protective agent for DNA damage and cell proliferation, the two molecular events in oncogenesis. Plant extract was prepared by hot extraction using methanol as solvent. Various chemical tests were performed to collectively understand its free radical-scavenging activity, including 2,2-diphenyl-1-picrylhydrazyl, nitric oxide, potassium ferricyanide, hydroxyl radical, and hydrogen peroxide assays. Free radicals used or produced in these assays represent those that cause DNA damages in cells. The plant extract showed free radical-scavenging activity in all the tests and was as effective as standard drugs. Cancer cell lines such as Dalton’s lymphoma ascites (DLA) and Chinese hamster lung carcinoma (V79) cells were used for anticancer assays. The plant extract showed anticancer activity only on lymphoma cells; but there was no discernible inhibition on carcinoma. The study shows that S. acmella is a promising source of therapeutic agent in the prevention of cancer development and DNA damage. Mon, 01 Apr 2019 00:00:00 GMT http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/797 2019-04-01T00:00:00Z http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/796 Title: Some Phytochemical, Antimicrobial and Anticancer Tests for an Aqueous extract of Acmella oleracea Authors: Lalchhandama, K Abstract: Acmella oleracea (family Asteraceae) is known for its diverse applications such as food supplement, vegetable, antioxidant, antineoplastic, antimicrobial, ornamental plant and pig fodder. We prepared an aqueous extract from which the presence of alkaloids, tannins and saponins were detected. These chemical groups have been known for their wide-ranging therapeutic properties. The antibacterial activity was tested on Gram-negative bacteria such as Klebsiella pneumoniae, Pseudomonas aeruginosa and Escherichia coli, and a Gram-positive species Bacillus subtilis. The plant extract showed no activity compared to that of the standard antibiotic tetracycline. Cytotoxic activity was also studied on cancer cell lines. We found an inverse relationship between treatment concentration and percent inhibition for HeLa (ATCC® CCL-2™) and V79 (ATCC® CCL-93™), however, the plant extract showed negligible effect on Dalton’s lymphoma ascites even at the highest concentration tested. The lower degree of inhibitions observed at higher concentrations for HeLa and V79 suggests that the plant extract might confer proliferative effect rather than antimitogenic effects on the cancer cells. The results indicate that A. oleracea has unique medicinal properties, which in turn provide the rationale for further investigations Tue, 06 Aug 2019 00:00:00 GMT http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/796 2019-08-06T00:00:00Z