DSpace Collection:http://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/6292026-05-01T05:03:22Z2026-05-01T05:03:22ZIsolation and Characterization of Endophytes Bacterial Strains of Momordica charantia L. and Their Possible Approach in Stress ManagementSingh, Prasant Kumarhttp://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/6372024-06-13T05:13:18Z2022-01-26T00:00:00ZTitle: Isolation and Characterization of Endophytes Bacterial Strains of Momordica charantia L. and Their Possible Approach in Stress Management
Authors: Singh, Prasant Kumar
Abstract: In the present study, eight endophytic bacterial strains, namely Bacillus licheniformis R1,
Bacillus sp. R2, Agrobacterium tumefaciens R6, uncultured bacterium R11, Bacillus subtilis RS3, Bacillus
subtilis RS6, uncultured bacterium RS8 and Lysinibacillus fusiformis RS9, were isolated from the root of
Momordica charantia L. All the strains, except R6 exhibited positive for IAA production, siderophore
production, and phosphate solubilization during plant growth-promoting traits analysis. Strains
invariably utilized glucose and sucrose as a carbon source during substrate utilization, while yeast
extract, ammonium sulphate, ammonium chloride, glycine, glutamine, and isoleucine as nitrogen
sources. In addition, Spectinomycin was found as the most effective during antibiotic sensitivity TEST,
followed by Chloramphenicol, Erythromycin, Rifampicin and Kanamycin, while Polymixin B was
found least effective, while strains R1, R6, and RS8 were sensitive to all the antibiotics. Strains R1 and
RS6 were able to withstand tolerance up to 10% of NaCl. The strains showing resistance against broadspectrum
antibiotics, especially chloramphenicol, can be used in hospital waste management. In
addition, strains with a tolerance of 10 % of NaCl can improve plant growth in the saline affected area.2022-01-26T00:00:00ZNitrogen Deprivation in Fremyella diplosiphon Augments Lipid Production without A ecting GrowthSingh, Prasant Kumarhttp://pucir.inflibnet.ac.in:8080/jspui/handle/123456789/6302024-06-12T10:44:32Z2020-10-27T00:00:00ZTitle: Nitrogen Deprivation in Fremyella diplosiphon Augments Lipid Production without A ecting Growth
Authors: Singh, Prasant Kumar
Abstract: Metabolic products such as lipids and proteins produced in cyanobacteria represent an
excellent source of biomass and do not compete with agricultural land use unlike soybean and
corn. Given their potential use as novel materials for biodiesel production, we aimed to explore
the e ect of cultivation period and nitrogen concentration on the growth rate and lipid content of
Fremyella diplosiphon, a model cyanobacterium. In this study, F. diplosiphon grown in BG11/HEPES
medium supplemented with 1.5 g L1 sodium nitrate (NaNO3) for 7, 10, 15, and 20 days were compared
to the untreated control in media amended with 0.25, 0.5, and 1.0 g L1 NaNO3. Cultures were
inoculated in liquid media and grown under continuous fluorescent light in an orbital incubator shaker,
and extracted lipids subjected to gravimetric analysis and gas chromatography-mass spectroscopy
to determine the best culture conditions for lipid production. Our results demonstrated that a
reduction in nitrogen concentration had no significant e ect on the growth rate across all cultivation
periods; however, the accumulation of total lipid content was significantly influenced by nitrogen
concentration. A maximum lipid production (40%) with no reduction in growth was observed in
10-day old cultures in a BG11/HEPES medium supplemented with 1.0 g L1 NaNO3. Fatty acid
methyl ester composition of transesterified lipids demonstrated high amounts of methyl palmitate
(50–70%) followed by methyl octadecenoate (17–30%) in the accumulated lipids at all treatments.
Trace quantities of methyl dodecanoate, methyl hexadecanoate, methyl octadecanoate, and methyl
octadecadienoate (1–8%) were also observed in all tested samples, indicating that nitrogen deprivation
in culture media increases lipid production without a ecting grow2020-10-27T00:00:00Z